1 Scope
This standard specifies the high performance liquid chromatographic method with immunoaffinity column clean-up for the determination of ochratoxin A content in foods.
This standard is applicable to the determination of ochratoxin A content in grain and grain products, liquor, soy sauce, vinegar, sauce and sauce products.
The detection limit by the method specified in this standard: that of grain and grain products is 1.0μg/kg, that of liquor is 0.1μg/kg and that of soy sauce, vinegar, sauce and sauce products is 0.5μg/kg.
2 Normative References
The following standard contains provisions which, through reference in this standard, constitute provisions of this standard. For dated reference, subsequent amendments to (excluding corrections to) or revisions of, any of these publications do not apply. However, all parties coming to an agreement according to this Standard are encouraged to study whether the latest editions of these documents apply. For undated reference, the latest edition of the normative documents referred to applies.
GB/T 6682 Water for Laboratory Use - Specifications and Test Method (GB/T 6682-1992, 3696: 1987 MOD)
3 Method Summary
Extract the ochratoxin A in the specimen with extract, purify it with immunoaffinity column, then determine it with high performance liquid chromatography-ultraviolet detector and quantitate it by external standard method.
4 Reagents and Materials
Unless otherwise specified, analytically pure reagents and Grade 1 water (specified in GB/T 6682) are adopted.
4.1 Methanol: chromatographically pure.
Foreword i
1 Scope
2 Normative References
3 Method Summary
4 Reagents and Materials
5 Apparatuses
6 Analytical Procedures
7 Result Calculation
8 Recovery Rate
9 Repeatability
Annex A (Informative) Liquid Chromatogram of Ochratoxin A Standard
