Method for the determination of α-trenbolone, β-trenbolone residues in bovine muscle, liver and kidney -
LC-UV method and LC-MS-MS method
1 Scope
This standard specifies the LC-UV method and LC-MS-MS method for the determination of α-trenbolone, β-trenbolone residues in bovine muscle, liver and kidney.
This standard is applicable to the determination of α-trenbolone, β-trenbolone residues in bovine muscle, liver and kidney.
For the method detection limit of this standard: the detection limits of LC-UV method and LC-MS-MS method for the determination of α-trenbolone, β-trenbolone are both 2μg/kg.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this standard. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.
GB/T 6379.1 Accuracy(trueness and precision)of measurement methods and results - Part 1: General principles and definitions (GB/T 6379.1-2004; ISO 5725-1: 1994, IDT)
GB/T 6379.2 Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method (GB/T 6379.2-2004; ISO 5725-2:1994, IDT)
GB/T 6682 Water for analytical laboratory use - Specification and test methods (GB/T 6682-1992; neq ISO 3696:1987)
Foreword i
1 Scope
2 Normative references
3 Principle
4 Reagents and materials
5 Apparatus
6 Preparation and preservation of specimen
7 Determination procedures
8 Result calculation
9 Precision
Annex A (Informative) Liquid chromatogram of reference-materials
Annex B (Informative) Recovery rate
